Journal:

Article Title: Intracellular Butyryl Phosphate and Acetyl Phosphate Concentrations in Clostridium acetobutylicum and Their Implications for Solvent Formation

doi: 10.1128/AEM.71.1.530-537.2005

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: All bacterial strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristics a Source or reference b C. acetobutylicum strains ATCC 824 Wild type ATCC PJC4BK Em r , butyrate kinase mutant 12 PJC4PTA Em r , phosphate acetyltransferase mutant 12 M5 pSOL1 − 7 E. coli TOP10 Invitrogen Plasmids pJC7 Ap r , BK gene 4 pTrcHis-TOPO Ap r , overexpression vector Invitrogen pTrcHis-BK Ap r , BK overexpression This study Open in a separate window a Ap, ampicillin; Em, erythromycin. b ATCC, American Type Culture Collection, Manassas, Va; Invitrogen, Invitrogen Corporation, Carlsbad, Calif. Bacterial strains and plasmids Chemicals.

Techniques: Plasmid Preparation, Mutagenesis, Over Expression